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To investigate the antibacterial activities of 53 species of mushrooms for Gram-positive bacteria, bioactive substances from mushrooms were extracted with petroleum ether, 80% ethanol, and distilled water. The 14 extracts of 12 species of mushrooms, largely with 8096 ethanol, showed antibacterial activities against 6 species of Gram-positive bacteria selectively. Especially, the extracts of Thylopilus PS-5, Pulveroboletus PS-7, and Russula PS-9 showed various antibacterial activities against several bacteria. Minimal inhibitory concentration (MIC) of the 80% ethanol extracts from Pulverobolerus PS-7 and Boletus PS-8 was 300 ㎍/㎖ against Bscillus subtilis, and that of the 80% ethanol extracts from Trichaptium PS-3 was 500 ㎍/㎖ against Micrococcus luteus. MIC of other extracts with antibacterial activities was above 1000 ㎍/㎖.
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